Electroejaculation can be done in a chute with untrained bulls. A drop-down panel gives access to the extended penis. Semen is generally collected with the aid of a so-called loving cup, a long handle with a ring at the end in which a funnel is suspended. A sterile graduated test tube is attached to the stem of the funnel.
Some bulls lie down in the squeeze chute during the electroejaculation procedure. It is generally better to apply minimal squeezing allowing the bull to support his own weight. May be more common in Bos indicus bulls.
Some bulls, especially those of Bos indicus breeding, go down during the process of electroejaculation. To maintain access to the penis for collection of the semen sample, a heavy belt is sometimes used to prevent the bull from lying down. Bulls are more likely to go down when they are squeezed tightly in the chute.
During warm weather a test tube and a small funnel can be used to collect the semen sample. A PVC cylinder with a handle can be used to hold the test tube.
This French mobile phantom has a seat under it in which a person can sit and hold the artificial vagina for the bull to serve. The phantom is covered with a hide from a Normandie cow.
Semen collection with an artificial vagina is routinely done in bull studs, or artificial insemination centers. The process requires a teaser animal or a phantom and a trained bull. As shown here it can be done under field conditions with tractable animals.
Sample from a bull with seminal vesiculitis. Note the precipitate of pus in the bottom (tip) of the tube. Despite the seminal vesiculitis and the presence of pus in the semen, the motility and morphology were normal.
Large open top liquid nitrogen (LN2) vat for processing and storing semen. 0.25 cc straws are being frozen in LN2 vapor on racks set on a screen above the LN2. Diameter of the opening of the vat is approximately 1 meter.
The two large tall cylinders are pressurized liquid nitrogen (LN2) storage tanks. The large stainless steel storage tank is in essence a large thermos bottle (the LN2 is not under pressure).
The spermatozoon with normal head shape shows a small folded area of the acrosomal ridge. The other spermatozoon has a more severe acrosomal abnormality.
A bent tail is considered a minor defect which may have occurred during improper handling of the semen sample. This abnormality is sometimes referred to as a kinked tail or the distal midpiece reflex.