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These oocytes have several intact layers of cumulus cells. They were isolated by the process of slashing of slaughterhouse ovaries. (Cumulus Oocyte Complex = COC)
Block J (2007)
The number of layers is less than three. These COC can still be used, but the rate of development may be lower. (Cumulus Oocyte Complex = COC)
Oocytes right after harvesting. The cumulus cells had already expanded. These would be excluded from the process of in-vitro fertilization (IVF). (Cumulus Oocyte Complex = COC)
Debris shown right after slashing. Oocytes can be identified at 10 to 25 X, aspirated, washed and transfered to the maturation medium.
Oocytes were removed from the search plate after slashing and are now ready for washing to remove the remaining smaller debris.
Oocytes after the third and final wash. They are now ready to be placed into the maturation medium.
Very nice mature oocytes with several layers of cumulus cells and homogeneous appearance of the cytoplasm (after 22 to 24 hours of incubation).
Mature oocytes after 22 to 24 hours of incubation shown in a microdrop of maturation medium.
Oocytes after eight hours of co-incubation with spermatozoa. Cumulus cells have begun to separate from the oocyte proper. The next step is vortexing to remove the attached cumulus cells.
Oocytes eight hours after insemination / exposure to sperm. Cumulus cells were removed by vortexing for 3 to 5 minutes at high speed.
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