The Visual Guide to
Equine Reproduction
Male Reproductive System: Semen

Cool Shipped Semen.
Shipped semen cooled to 5 degrees Centigrade for 12 hours in the shipping container and is kept at 21 degrees Centigrade (room temperature) during the evaluation process in the laboratory, prior to insemination.
Asbury AC (1987)

Sample for Sperm Count.
Preparation of a samples for a sperm count with a hemocytometer.
Asbury AC (1987)

Warming Tray.
All slides and coverslips are maintained at 37 degrees Centigrade on a warming tray.
Asbury AC (1988)

Staining for Morphology.
A drop of neat semen is placed on a warm microscope slide. It is mixed with stain before a smear is made by spreading the drop with the end of a second slide.
Asbury AC (1988)

Microscopic Evaluation.
Sperm motility is evaluated at 200x Morphology is evaluated at 1000x. Shown is a double headed microscope used for teaching purposes. This scope also has a camera attached for direct transmission to a monitor screen.
Asbury AC (1988)

Sperm Count.
A sperm count is made with the aid of a hemocytometer.
Asbury AC (1988)

Primordial Cells and Abaxial Tails.
This stained semen smear shows two primordial cells (dark green) and several abaxial (offset) tails.
Drost M (1980)